In:
Current Pharmaceutical Design, Bentham Science Publishers Ltd., Vol. 24, No. 24 ( 2018-11-08), p. 2883-2889
Abstract:
Atherosclerosis is regarded as a chronic inflammatory disease associated with changes in the innate
immune system functioning and cytokine disturbances. Local inflammation in the arterial wall is an important component in the development and growth of atherosclerotic plaques. Inside the lesions, both pro- and antiinflammatory
cytokines were detected, highlighting the complexity of the atherosclerotic process. However, little is known about the expression of these signaling molecules in early human atherosclerotic lesions. In this study,
we explored localization of a pro-inflammatory cytokine, tumor necrosis factor-α (TNFα), and anti-inflammatory
chemokine, C-C motif chemokine ligand 18 (CCL18), in the arterial wall of human aorta. We noticed differences in the intensity of staining for TNFα and CCL18 in atherosclerotic lesions and grossly normal areas, as well as
differences in their localization. While CCL18 prevailed in the areas close to the aortic lumen, TNFα was localized in deeper layers of the intima. We next studied the expression of TNFα and CCL18 mRNA in lesions corresponding
to different stages of atherosclerosis progression and found that it was maximal in lipofibrous plaques that are most enriched in lipids. To test the hypothesis that cytokine expression can be associated with lipid accumulation,
we studied the TNFα and CCL18 expression profiles in primary human monocyte-derived macrophages after inducing lipid accumulation by incubating cultured cells with atherogenic LDL. We found that intracellular
cholesterol accumulation was associated with upregulation of both TNFα and CCL18, confirming our hypothesis. These results encourage further investigation of cytokine expression in human atherosclerotic lesions
and its role in the atherosclerosis progression.
Type of Medium:
Online Resource
ISSN:
1381-6128
DOI:
10.2174/1381612824666180911120726
Language:
English
Publisher:
Bentham Science Publishers Ltd.
Publication Date:
2018
detail.hit.zdb_id:
1304236-1
SSG:
15,3
