In:
Journal of Robotics and Mechatronics, Fuji Technology Press Ltd., Vol. 19, No. 5 ( 2007-10-20), p. 519-523
Abstract:
Evaluating cell activity and functions in different-sized cell chambers requires multiscale sensing. We have been developing multiscale biosensing applied from 10 µm to 1 mm. We measured mouse IgG in micro wells using a high-resolution two-dimensional surface plasmon resonance (SPR) imaging affinity sensor. This sensor uses high refractive optics, a 1X to 7X microscopic lens, and a cooled CCD camera. The micro-well array was prepared with a PDMS film on gold sensor film. Protein A immobilized on sensor film was used for IgG recognition. SPR sensitivity was dramatically decreased with 10 and 8.5 µm microwells. To improve sensor sensitivity, we optimized the sensor’s measurement angle and exposure time, enabling mouse IgG to be detected in wells of 1 mm, 30 µm, and 10 µm using the same 2D-SPR imaging sensor and measurement protocol. These results show the feasibility of multiscale biosensing use in antibody production in a micro well or a cell chamber.
Type of Medium:
Online Resource
ISSN:
1883-8049
,
0915-3942
DOI:
10.20965/jrm.2007.p0519
Language:
English
Publisher:
Fuji Technology Press Ltd.
Publication Date:
2007
detail.hit.zdb_id:
2587053-1
detail.hit.zdb_id:
1021878-6
SSG:
31