In:
PLOS ONE, Public Library of Science (PLoS), Vol. 18, No. 5 ( 2023-5-30), p. e0285724-
Abstract:
Lung transplantation is the only curative option for end-stage chronic respiratory diseases. However the survival rate is only about 50% at 5 years. Although experimental evidences have shown that innate allo-responses impact on the clinical outcome, the knowledge of the involved mechanisms involved is limited. We established a cross-circulatory platform to monitor the early recruitment and activation of immune cells in an extracorporeal donor lung by coupling blood perfusion to cell mapping with a fluorescent marker in the pig, a commonly-used species for lung transplantation. The perfusing pig cells were easily detectable in lung cell suspensions, in broncho-alveolar lavages and in different areas of lung sections, indicating infiltration of the organ. Myeloid cells (granulocytes and monocytic cells) were the dominant recruited subsets. Between 6 and 10 h of perfusion, recruited monocytic cells presented a strong upregulation of MHC class II and CD80/86 expression, whereas alveolar macrophages and donor monocytic cells showed no significant modulation of expression. This cross-circulation model allowed us to monitor the initial encounter between perfusing cells and the lung graft, in an easy, rapid, and controllable manner, to generate robust information on innate response and test targeted therapies for improvement of lung transplantation outcome.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0285724
DOI:
10.1371/journal.pone.0285724.g001
DOI:
10.1371/journal.pone.0285724.g002
DOI:
10.1371/journal.pone.0285724.g003
DOI:
10.1371/journal.pone.0285724.g004
DOI:
10.1371/journal.pone.0285724.g005
DOI:
10.1371/journal.pone.0285724.s001
DOI:
10.1371/journal.pone.0285724.s002
DOI:
10.1371/journal.pone.0285724.s003
DOI:
10.1371/journal.pone.0285724.s004
DOI:
10.1371/journal.pone.0285724.s005
DOI:
10.1371/journal.pone.0285724.s006
DOI:
10.1371/journal.pone.0285724.s007
DOI:
10.1371/journal.pone.0285724.s008
DOI:
10.1371/journal.pone.0285724.s009
DOI:
10.1371/journal.pone.0285724.s010
DOI:
10.1371/journal.pone.0285724.r001
DOI:
10.1371/journal.pone.0285724.r002
DOI:
10.1371/journal.pone.0285724.r003
DOI:
10.1371/journal.pone.0285724.r004
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2023
detail.hit.zdb_id:
2267670-3