In:
PLOS ONE, Public Library of Science (PLoS), Vol. 16, No. 3 ( 2021-3-8), p. e0248073-
Kurzfassung:
Obesity is a major risk factor for various chronic diseases such as diabetes, cardiovascular disease, and cancer; hence, there is an urgent need for an effective strategy to prevent this disorder. Currently, the anti-obesity effects of food ingredients are drawing attention. Therefore, we focused on carob, which has high antioxidant capacity and various physiological effects, and examined its anti-obesity effect. Carob is cultivated in the Mediterranean region, and its roasted powder is used as a substitute for cocoa powder. We investigated the effect of carob pod polyphenols (CPPs) on suppressing increases in adipose tissue weight and adipocyte hypertrophy in high fat diet-induced obesity model mice, and the mechanism by which CPPs inhibit the differentiation of 3T3-L1 preadipocytes into adipocytes in vitro . In an in vivo experimental system, we revealed that CPPs significantly suppressed the increase in adipose tissue weight and adipocyte hypertrophy. Moreover, in an in vitro experimental system, CPPs acted at the early stage of differentiation of 3T3-L1 preadipocytes and suppressed cell proliferation because of differentiation induction. They also suppressed the expression of transcription factors involved in adipocyte differentiation, thereby reducing triacylglycerol synthesis ability and triglycerol (TG) accumulation. Notably, CPPs regulated CCAAT/enhancer binding protein (C/EBP)β, which is expressed at the early stage of differentiation, at the posttranscriptional level. These results demonstrate that CPPs suppress the differentiation of adipocytes through the posttranscriptional regulation of C/EBPβ and may serve as an effective anti-obesity compound.
Materialart:
Online-Ressource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0248073
DOI:
10.1371/journal.pone.0248073.g001
DOI:
10.1371/journal.pone.0248073.g002
DOI:
10.1371/journal.pone.0248073.g003
DOI:
10.1371/journal.pone.0248073.g004
DOI:
10.1371/journal.pone.0248073.g005
DOI:
10.1371/journal.pone.0248073.g006
DOI:
10.1371/journal.pone.0248073.g007
DOI:
10.1371/journal.pone.0248073.g008
DOI:
10.1371/journal.pone.0248073.g009
DOI:
10.1371/journal.pone.0248073.g010
DOI:
10.1371/journal.pone.0248073.g011
DOI:
10.1371/journal.pone.0248073.g012
DOI:
10.1371/journal.pone.0248073.g013
DOI:
10.1371/journal.pone.0248073.g014
DOI:
10.1371/journal.pone.0248073.t001
DOI:
10.1371/journal.pone.0248073.t002
DOI:
10.1371/journal.pone.0248073.s001
DOI:
10.1371/journal.pone.0248073.r001
DOI:
10.1371/journal.pone.0248073.r002
DOI:
10.1371/journal.pone.0248073.r003
DOI:
10.1371/journal.pone.0248073.r004
Sprache:
Englisch
Verlag:
Public Library of Science (PLoS)
Publikationsdatum:
2021
ZDB Id:
2267670-3