In:
PLOS Computational Biology, Public Library of Science (PLoS), Vol. 17, No. 9 ( 2021-9-22), p. e1009381-
Abstract:
The increased complexity of synthetic microbial biocircuits highlights the need for distributed cell functionality due to concomitant increases in metabolic and regulatory burdens imposed on single-strain topologies. Distributed systems, however, introduce additional challenges since consortium composition and spatiotemporal dynamics of constituent strains must be robustly controlled to achieve desired circuit behaviors. Here, we address these challenges with a modeling-based investigation of emergent spatiotemporal population dynamics using cell-length control in monolayer, two-strain bacterial consortia. We demonstrate that with dynamic control of a strain’s division length, nematic cell alignment in close-packed monolayers can be destabilized. We find that this destabilization confers an emergent, competitive advantage to smaller-length strains—but by mechanisms that differ depending on the spatial patterns of the population. We used complementary modeling approaches to elucidate underlying mechanisms: an agent-based model to simulate detailed mechanical and signaling interactions between the competing strains, and a reductive, stochastic lattice model to represent cell-cell interactions with a single rotational parameter. Our modeling suggests that spatial strain-fraction oscillations can be generated when cell-length control is coupled to quorum-sensing signaling in negative feedback topologies. Our research employs novel methods of population control and points the way to programming strain fraction dynamics in consortial synthetic biology.
Type of Medium:
Online Resource
ISSN:
1553-7358
DOI:
10.1371/journal.pcbi.1009381
DOI:
10.1371/journal.pcbi.1009381.g001
DOI:
10.1371/journal.pcbi.1009381.g002
DOI:
10.1371/journal.pcbi.1009381.g003
DOI:
10.1371/journal.pcbi.1009381.g004
DOI:
10.1371/journal.pcbi.1009381.g005
DOI:
10.1371/journal.pcbi.1009381.g006
DOI:
10.1371/journal.pcbi.1009381.g007
DOI:
10.1371/journal.pcbi.1009381.g008
DOI:
10.1371/journal.pcbi.1009381.s001
DOI:
10.1371/journal.pcbi.1009381.s002
DOI:
10.1371/journal.pcbi.1009381.s003
DOI:
10.1371/journal.pcbi.1009381.s004
DOI:
10.1371/journal.pcbi.1009381.s005
DOI:
10.1371/journal.pcbi.1009381.s006
DOI:
10.1371/journal.pcbi.1009381.s007
DOI:
10.1371/journal.pcbi.1009381.s008
DOI:
10.1371/journal.pcbi.1009381.s009
DOI:
10.1371/journal.pcbi.1009381.s010
DOI:
10.1371/journal.pcbi.1009381.s011
DOI:
10.1371/journal.pcbi.1009381.s012
DOI:
10.1371/journal.pcbi.1009381.s013
DOI:
10.1371/journal.pcbi.1009381.s014
DOI:
10.1371/journal.pcbi.1009381.s015
DOI:
10.1371/journal.pcbi.1009381.s016
DOI:
10.1371/journal.pcbi.1009381.s017
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2021
detail.hit.zdb_id:
2193340-6