In:
Journal of Cell Science, The Company of Biologists, Vol. 111, No. 20 ( 1998-01-15), p. 3109-3118
Abstract:
Proteins of the armadillo family are involved in diverse cellular processes in higher eukaryotes. Some of them, like armadillo, β-catenin and plakoglobins have dual functions in intercellular junctions and signalling cascades. Others, belonging to the importin-α-subfamily are involved in NLS recognition and nuclear transport, while some members of the armadillo family have as yet unknown functions. Here, we introduce the Saccharomyces cerevisiae protein Ye1013p as a novel armadillo (arm) repeat protein. The ORF Ye1013w was identified in the genome project on chromosome V (EMBL: U18530) and codes for an acidic protein of 578 residues with 8 central arm-repeats, which are closely related to the central repeat-domain of Xenopus laevis plakoglobin. We show that Ye1013p (Vac8p) is constitutively expressed in diploid and haploid yeasts and that it is not essential for viability and growth. However, the vacuoles of mutant cells are multilobular or even fragmented into small vesicles and the processing of aminopeptidase I, representing the cytoplasm-to-vacuole transport pathway, is strongly impaired. Consistent with these observations, subcellular fractionation experiments, immunolocalization and expression of green fluorescent protein (GFP) fusion proteins revealed that Ye1013p (Vac8p) is associated with the vacuolar membrane. Our data provide evidence for the involvement of an arm-family member in vacuolar morphology and protein targeting to the vacuole.
Type of Medium:
Online Resource
ISSN:
0021-9533
,
1477-9137
DOI:
10.1242/jcs.20.111.3109
Language:
English
Publisher:
The Company of Biologists
Publication Date:
1998
detail.hit.zdb_id:
219171-4
detail.hit.zdb_id:
1483099-1
SSG:
12
