In:
Development, The Company of Biologists, Vol. 110, No. 2 ( 1990-10-01), p. 471-475
Kurzfassung:
A mouse cDNA for the developmentally controlled, melanocyte-specific protein, tyrosinase-related protein 1 (TRP-1), was previously cloned and reported to show genetic linkage with the coat-colour locus brown (b) on mouse chromosome 4. The cDNA has been inserted into a retroviral vector derived from Moloney murine leukaemia virus, under the control of the human histone H4 promoter. This vector was used to infect melanocytes of the immortal line melan-b, which are homozygous for the b mutation and which display light brown pigmentation in culture. Infected cultures containing between 0.2 and 2 copies of provirus per cell displayed an altered phenotype: 20-50 % of cells now had the black to dark brown colour characteristic of cultured wild-type (Black, B/B) mouse melanocytes. Thus the TRP-1 gene complements the brown mutation. We conclude that TRP-1 is the product of the wild-type-b-locus.
Materialart:
Online-Ressource
ISSN:
0950-1991
,
1477-9129
DOI:
10.1242/dev.110.2.471
Sprache:
Englisch
Verlag:
The Company of Biologists
Publikationsdatum:
1990
ZDB Id:
2007916-3
SSG:
12
