In:
BMC Genomics, Springer Science and Business Media LLC, Vol. 24, No. 1 ( 2023-08-30)
Abstract:
The emergence and wide spread of carbapenemase-producing Enterobacteriaceae (CPE) poses a growing threat to global public health. However, clinically derived carbapenemase-producing Citrobacter causing multiple infections has rarely been investigated. Here we first report the isolation and comparative genomics of two bla NDM-5 carrying Citrobacter freundii ( C. freundii ) isolates from a patient with bloodstream and urinary tract infections. Results Antimicrobial susceptibility testing showed that both bla NDM-5 carrying C. freundii isolates were multidrug-resistant. Positive modified carbapenem inactivation method (mCIM) and EDTA-carbapenem inactivation method (eCIM) results suggested metallo-carbapenemase production. PCR and sequencing confirmed that both metallo-carbapenemase producers were bla NDM-5 positive. Genotyping and comparative genomics analyses revealed that both isolates exhibited a high level of genetic similarity. Plasmid analysis confirmed that the bla NDM-5 resistance gene is located on IncX3 plasmid with a length of 46,161 bp, and could successfully be transferred to the recipient Escherichia coli EC600 strain. A conserved structure sequence (IS Aba125 -IS 5 - bla NDM-5 - trpF -IS 26 - umuD -IS Kox3 ) was found in the upstream and downstream of the bla NDM-5 gene. Conclusions The data presented in this study showed that the conjugative bla NDM-5 plasmid possesses a certain ability to horizontal transfer. The dissemination of NDM-5-producing C. freundii isolates should be of close concern in future clinical surveillance. To our knowledge, this is the first study to characterize C. freundii strains carrying the bla NDM-5 gene from one single patient with multiple infections.
Type of Medium:
Online Resource
ISSN:
1471-2164
DOI:
10.1186/s12864-023-09579-9
Language:
English
Publisher:
Springer Science and Business Media LLC
Publication Date:
2023
detail.hit.zdb_id:
2041499-7
SSG:
12
