In:
BMC Microbiology, Springer Science and Business Media LLC, Vol. 6, No. 1 ( 2006-12)
Abstract:
It has been reported that some marine cyanophage are temperate and can be induced from a lysogenic phase to a lytic phase by different agents such as heavy metals. However, to date no significant reports have focused on the temperate nature of freshwater cyanophage/cyanobacteria. Previous experiments with cyanophage AS-1 and cyanobacteria Anacystis nidulans have provided some evidence that AS-1 may have a lysogenic life cycle in addition to the characterized lytic cycle. Results In this study, the possible temperate A. nidulans was treated with different concentrations of heavy metal-copper. CuSO 4 with concentrations of 3.1 × 10 -3 M, 3.1 × 10 -4 M, 3.1 × 10 -5 M and 3.1 × 10 -6 M were used to detect the induction of AS-1 from A. nidulans . The population of the host, unicellular cyanobacteria Anacystis nidulans , was monitored by direct count and turbidity while the amount of virus produced was derived from plaque forming units (PFU) by a direct plating method. The ratio of AS-1 release from A. nidulans was also determined. From these results it appears that AS-1 lysogenic phage can be induced by copper at concentrations from 3.1 × 10 -6 M to 3.1 × 10 -4 M. Maximal phage induction occurred at 6 hours after addition of copper, with an optimal concentration of 3.1 × 10 -6 M. Conclusion Cu 2+ is a significant inducer for lysogenic cyanobacterial cells and consequently would be a potential control agent in the cyanobacteria population in fresh water ecosystems.
Type of Medium:
Online Resource
ISSN:
1471-2180
DOI:
10.1186/1471-2180-6-17
Language:
English
Publisher:
Springer Science and Business Media LLC
Publication Date:
2006
detail.hit.zdb_id:
2041505-9
SSG:
12
