In:
Journal of Biomaterials and Tissue Engineering, American Scientific Publishers, Vol. 13, No. 2 ( 2023-02-01), p. 349-355
Abstract:
DOPC liposome properties of various sizes were analyzed via atomic force microscopy (AFM) and patch-clamp. The unilamellarity of small DOPC liposome (below 1 μ m) was confirmed via Cryogenic transmission electron microscopy (Cryo-TEM). Small DOPC liposome (below 1 μ m)
showed a bending modulus ( k bend ) ranging (between 10 −18 and 10 −20 J). The bending modulus value was a size dependence. In order words, it decreased as DOPC liposome size increased. For DOPC liposome (above 1 μ m), patch-clamp was
used to achieve electrically tight whole-cell configurations. Our result showed that the unilamellar DOPC liposome (above 1 μ m) exhibit RC circuit response property. It was similar to theoretical values ( τ = 4.52 ms) of the unilamellar liposome. In this study, the uniform
lamellarity of the DOPC liposome of various sizes was confirmed through electrical and mechanical properties of DOPC liposome.
Type of Medium:
Online Resource
ISSN:
2157-9083
DOI:
10.1166/jbt.2023.3263
Language:
English
Publisher:
American Scientific Publishers
Publication Date:
2023
detail.hit.zdb_id:
2717586-8