In:
Arteriosclerosis, Thrombosis, and Vascular Biology, Ovid Technologies (Wolters Kluwer Health), Vol. 15, No. 9 ( 1995-09), p. 1338-1344
Abstract:
Abstract The effects of paracetamol and sodium salicylate on the susceptibility of LDL to oxidative modification were studied. LDL was subjected to Cu 2+ -, azo compound–, or peripheral blood mononuclear cell–initiated oxidation in the absence and presence of paracetamol and salicylate. Paracetamol (100 μmol/L; 25 μg LDL/mL) reduced the rate of formation of conjugated dienes and the amount of conjugated dienes formed during Cu 2+ -induced oxidation by 67% and 58%, respectively. Paracetamol (400 μmol/L; 100 μg LDL/mL) reduced the generation of lipid peroxides during Cu 2+ -induced oxidation by 43% ( P 〈 .05), the relative electrophoretic mobility in agarose gels by 16% ( P 〈 .05), and the amount of oxidized LDL taken up by J774 macrophages by 22% ( P 〈 .05). Paracetamol (100 μmol/L; 100 μg LDL/mL) reduced the 2,2′-azobis-(2-amidinopropane hydrochloride)–initiated lipid peroxidation by 70% ( P 〈 .05) and the relative electrophoretic mobility by 34% ( P 〈 .05). Paracetamol (100 μmol/L; 100 μg LDL/mL) reduced the amount of lipid peroxides generated in LDL during mononuclear cell–mediated oxidation by 69% ( P 〈 .01) and the relative electrophoretic mobility by 38% ( P 〈 .01). In comparison, 10 μmol/L α-tocopherol reduced the amount of lipid peroxides formed during cellular LDL oxidation and the relative electrophoretic mobility by 52% and 65%, respectively ( P 〈 .05). In the absence of paracetamol, SOD and catalase inhibited the modification of LDL ( P 〈 .05), suggesting that superoxide anions and hydrogen peroxide might be involved in the cell-mediated modification pathway. In the presence of paracetamol, SOD showed no additional inhibitory effect. The 1,1-diphenyl-2-pikrylhydracyl radical–scavenging test showed that paracetamol itself was a free-radical scavenger. In contrast, sodium salicylate (25 to 4000 μmol/L) showed no free radical–scavenging property and failed to protect LDL against mononuclear cell–mediated oxidation. In conclusion, the results indicate that paracetamol, but not salicylate, protects LDL against Cu 2+ -induced, azo compound–initiated, and mononuclear cell–mediated oxidative modification in vitro and that this may be due to the radical scavenger capacity of paracetamol.
Type of Medium:
Online Resource
ISSN:
1079-5642
,
1524-4636
DOI:
10.1161/01.ATV.15.9.1338
Language:
English
Publisher:
Ovid Technologies (Wolters Kluwer Health)
Publication Date:
1995
detail.hit.zdb_id:
1494427-3
