In:
Acta Haematologica, S. Karger AG, Vol. 143, No. 3 ( 2020), p. 279-288
Abstract:
〈 b 〉 〈 i 〉 Background: 〈 /i 〉 〈 /b 〉 The aim of this study was to detect the expression of long noncoding RNA small nucleolar RNA host gene 18 ( 〈 i 〉 SNHG18 〈 /i 〉 ) and 〈 i 〉 〈 /i 〉 semaphorin 5A ( 〈 i 〉 SEMA5A 〈 /i 〉 ) genes in multiple myeloma (MM) patients and to explore the correlation of the expression of these genes with the clinical characteristics and prognosis of MM patients. 〈 b 〉 〈 i 〉 Methods: 〈 /i 〉 〈 /b 〉 Forty-seven newly diagnosed MM, 18 complete remission MM, 13 refractory/relapse MM, and 22 iron deficiency anemia (serving as control) samples were extracted at the Department of Hematology, Second Affiliated Hospital of Xian Jiaotong University between January 2015 and December 2016. The clinical features of the MM patients are summarized. Real-time quantitative PCR was performed to analyze the relative expression levels of the 〈 i 〉 SNHG18 〈 /i 〉 and 〈 i 〉 SEMA5A 〈 /i 〉 genes. The clinical characteristics and overall survival (OS) of the MM patients were statistically analyzed while measuring different levels of 〈 i 〉 SNHG18 〈 /i 〉 and 〈 i 〉 SEMA5A 〈 /i 〉 gene expression. At the same time, the correlation between the expression of 〈 i 〉 SNHG18 〈 /i 〉 and 〈 i 〉 SEMA5A 〈 /i 〉 was also analyzed. 〈 b 〉 〈 i 〉 Results: 〈 /i 〉 〈 /b 〉 The analysis confirmed that 〈 i 〉 SNHG18 〈 /i 〉 and its possible target gene 〈 i 〉 SEMA5A 〈 /i 〉 were both highly expressed in newly diagnosed MM patients. After analyzing the clinical significance of 〈 i 〉 SNHG18 〈 /i 〉 and 〈 i 〉 SEMA5A 〈 /i 〉 in MM patients, we found that the expression of 〈 i 〉 SNHG18 〈 /i 〉 and 〈 i 〉 SEMA5A 〈 /i 〉 was related to the Durie-Salmon (DS), International Staging System (ISS), and Revised International Staging System (R-ISS) classification systems, and the Mayo Clinic Risk Stratification for Multiple Myeloma (mSMART; 〈 i 〉 p 〈 /i 〉 & #x3c; 0.05). Moreover, we observed a significant difference in OS between the 〈 i 〉 SNHG18 〈 /i 〉 / 〈 i 〉 SEMA5A 〈 /i 〉 high expression group and the low expression group. We found a positive correlation between 〈 i 〉 SNHG18 〈 /i 〉 and 〈 i 〉 SEMA5A 〈 /i 〉 expression ( 〈 i 〉 r 〈 /i 〉 = 0.709, 〈 i 〉 p 〈 /i 〉 & #x3c; 0.01). Surprisingly, the expected median OS times of both the 〈 i 〉 SNHG18 〈 /i 〉 and 〈 i 〉 SEMA5A 〈 /i 〉 high expression groups were significantly decreased, which was in contrast to those of both the 〈 i 〉 SNHG18 〈 /i 〉 and 〈 i 〉 SEMA5A 〈 /i 〉 low expression groups and the single-gene high expression group ( 〈 i 〉 p 〈 /i 〉 & #x3c; 〈 i 〉 0.05 〈 /i 〉 ). 〈 b 〉 〈 i 〉 Conclusion: 〈 /i 〉 〈 /b 〉 High expression of both 〈 i 〉 SNHG18 〈 /i 〉 and 〈 i 〉 SEMA5A 〈 /i 〉 is associated with poor prognosis in patients with MM.
Type of Medium:
Online Resource
ISSN:
0001-5792
,
1421-9662
Language:
English
Publisher:
S. Karger AG
Publication Date:
2020
detail.hit.zdb_id:
1481888-7
detail.hit.zdb_id:
80008-9
