In:
International Archives of Allergy and Immunology, S. Karger AG, Vol. 151, No. 4 ( 2010), p. 318-330
Abstract:
〈 i 〉 Background: 〈 /i 〉 Seminal vesicle protein number 4 (SV-IV) is a small, basic, multifunctional, intrinsically disordered secretory protein synthesized in large amounts by rat seminal vesicle epithelium under androgen transcriptional control. SV-IV-immunorelated proteins occur in other rat tissues and in humans. 〈 i 〉 Methods: 〈 /i 〉 The in vitro effect of SV-IV on human Fc & #917;RI+ cells was investigated by standard immunologic, biochemical and molecular biology procedures. 〈 i 〉 Results: 〈 /i 〉 SV-IV-induced histamine release from human basophils and lung mast cells without any influence on leukotriene C 〈 sub 〉 4 〈 /sub 〉 release and cell migration. The histamine release rate was slower compared with that induced by anti-IgE, the temperature dependence of the event being similar. SV-IV-induced histamine release was Ca 〈 sup 〉 2+ 〈 /sup 〉 -dependent, suggesting a physiological interaction of the protein with Fc & #917;RI+ cells. SV-IV and anti-IgE acted synergistically on the histamine release. SV-IV did not induce de novo synthesis of cytokines and growth factors (transforming growth factor-β 〈 sub 〉 1 〈 /sub 〉 , interleukin-10, interleukin-13, tumor necrosis factor-α, vascular endothelial growth factor A) in Fc & #917;RI+ cells. 〈 i 〉 Conclusions: 〈 /i 〉 SV-IV protein induces in human Fc & #917;RI+ cells the release of histamine, a proinflammatory, antiapoptotic and immunosuppressive biogenic amine. These data: (1) are consistent with the antiapoptotic and immunosuppressive properties of SV-IV; (2) confirm a regulatory feature of SV-IV on mammal inflammatory reactivity by either inhibiting the arachidonate cascade pathway or stimulating proinflammatory cytokine release from lymphocyte/monocytes and histamine from Fc & #917;RI+ cells; (3) raise the possibility of a protective role of SV-IV on implanting hemiallogenic blastocysts against maternal reactive oxygen species and immunological attacks at the uterine implantation site.
Type of Medium:
Online Resource
ISSN:
1018-2438
,
1423-0097
Language:
English
Publisher:
S. Karger AG
Publication Date:
2010
detail.hit.zdb_id:
1108932-5
detail.hit.zdb_id:
1482722-0
