In:
Cancer Research, American Association for Cancer Research (AACR), Vol. 71, No. 8_Supplement ( 2011-04-15), p. 3051-3051
Abstract:
Background: The active metabolite of vitamin D, 1, 25-dihydroxyvitamin D3 (calcitriol), inhibits the growth of several types of human cancer cells in vitro, but its therapeutic use is limited because it causes hypercalcemia. Among its analogs, 19-nor-1, 25-dihydroxyvitamin D2 (paricalcitol) induces fewer calcemic effects and exhibits an activity that is equipotential to that of calcitriol in several in vivo and in vitro systems. We investigated the antitumor activity and mechanism of action of paricalcitol in gastric cancer cells. Methods: We examined the effects of paricalcitol on cell proliferation, cell cycle, apoptosis, and inflammation/immunity in the human gastric cancer cell lines AGS, SNU719, and MKN45. Results: In vitro treatment with paricalcitol was associated with the following: (1) a dose-dependent increase in vitamin D receptor (VDR) protein expression; (2) inhibited gastric cell growth and induced apoptosis in AGS, SNU719, and MKN45 cell lines; (3) increased expression of p21 and p27 and decreased expression of CDK2; (4) increased expression of the caspase-3 cleaved protein and decreased expression of the anti-apoptotic Bcl-2 and Bax proteins; (5) promotion of early and late apoptosis; and (6) decreased expression of STAT-3, JAK2, COX2, and NF-κB. Conclusion: The low-calcemic vitamin D analog, paricalcitol, exhibits anticancer activity against gastric cancer cells, and its mechanism of action may be mediated through the VDR. Paricalcitol is a promising agent for the prevention and treatment of gastric cancer. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 3051. doi:10.1158/1538-7445.AM2011-3051
Type of Medium:
Online Resource
ISSN:
0008-5472
,
1538-7445
DOI:
10.1158/1538-7445.AM2011-3051
Language:
English
Publisher:
American Association for Cancer Research (AACR)
Publication Date:
2011
detail.hit.zdb_id:
2036785-5
detail.hit.zdb_id:
1432-1
