In:
American Journal of Physiology-Heart and Circulatory Physiology, American Physiological Society, Vol. 294, No. 4 ( 2008-04), p. H1700-H1707
Abstract:
The central nervous system (CNS) pericytes play an important role in brain microcirculation. Na + /H + exchanger isoform 1 (NHE1) has been suggested to regulate the proliferation of nonvascular cells through the regulation of intracellular pH, Na + , and cell volume; however, the relationship between NHE1 and intracellular Ca 2+ , an essential signal of cell growth, is still not known. The aim of the present study was to elucidate the role of NHE1 in Ca 2+ signaling and the proliferation of human CNS pericytes. The intracellular Ca 2+ concentration was measured by fura 2 in cultured human CNS pericytes. The cells showed spontaneous Ca 2+ oscillation under quasi-physiological ionic conditions. A decrease in extracellular pH or Na + evoked a transient Ca 2+ rise followed by Ca 2+ oscillation, whereas an increase in pH or Na + did not induce the Ca 2+ responses. The Ca 2+ oscillation was inhibited by an inhibitor of NHE in a dose-dependent manner and by knockdown of NHE1 by using RNA interference. The Ca 2+ oscillation was completely abolished by thapsigargin. The proliferation of pericytes was attenuated by inhibition of NHE1. These results demonstrate that NHE1 regulates Ca 2+ signaling via the modulation of Ca 2+ release from the endoplasmic reticulum, thus contributing to the regulation of proliferation in CNS pericytes.
Type of Medium:
Online Resource
ISSN:
0363-6135
,
1522-1539
DOI:
10.1152/ajpheart.01203.2007
Language:
English
Publisher:
American Physiological Society
Publication Date:
2008
detail.hit.zdb_id:
1477308-9
SSG:
12
