In:
American Journal of Physiology-Gastrointestinal and Liver Physiology, American Physiological Society, Vol. 282, No. 6 ( 2002-06-01), p. G1024-G1034
Abstract:
γ/δ T cells might play an important role in autoimmune conditions like inflammatory bowel disease (IBD). In the present study, we characterized the T cell receptor (TCR)-δ repertoire by complementarity determining region 3 (CDR3) spectratyping in the inflamed and noninflamed mucosa and in the peripheral blood of subjects with Crohn's disease and ulcerative colitis. In contrast to previously published data about α/β T cells, we rarely found oligoclonal expansions of γ/δ T cells specific only for the inflamed mucosa. The same dominant γ/δ T cell expansions were also present in the noninflamed colon. Furthermore, the peripheral γ/δ TCR repertoire was oligoclonal but clearly distinct from that in the inflamed intestine. Thus our results do not support a role for antigen-specific γ/δ T cells in IBD, and dominant γ/δ T cells of the peripheral blood are not likely to be derived from the inflamed gut. However, in several patients, the TCR-δ-repertoire was highly diversified, whereas in others we observed a loss of dominant γ/δ T cell clones when inflamed and noninflamed mucosa were compared. In conclusion, those changes indicate that γ/δ T cells might play an important role in a subset of patients with IBD.
Type of Medium:
Online Resource
ISSN:
0193-1857
,
1522-1547
DOI:
10.1152/ajpgi.00224.2001
Language:
English
Publisher:
American Physiological Society
Publication Date:
2002
detail.hit.zdb_id:
1477329-6
SSG:
12
