In:
The American Journal of Chinese Medicine, World Scientific Pub Co Pte Ltd, Vol. 42, No. 04 ( 2014-01), p. 921-934
Abstract:
Humulus scandens, rich in flavonoids, is a traditional Chinese medicine. It is widely used in China to treat tuberculosis, dysentery and chronic colitis. In this study, the major active faction of Humulus scandens (H.S) was prepared. Then, its immunosuppressive effects and underlying mechanisms on T cell activation were investigated in vitro and in vivo. Results showed that H.S significantly inhibited the proliferation of splenocytes induced by concanavalin A, lipopolysaccharides, and mixed-lymphocyte reaction in vitro. Additionally, H.S could dramatically suppress the proliferation and interferon-γ (IFN-γ) production from T cells stimulated by anti-CD3 and anti-CD28. Flow cytometric results confirmed that H.S could suppress the differentiation of IFN-γ-producing type 1 helper T cells (Th1). Furthermore, using ovalbumin immunization-induced T cell reaction and CD4 + T-cell-mediated delayed type hypersensitivity reaction, H.S the immunosuppressive effects of H.S was also demonstrated in vivo. Western blot results showed that H.S could impede the activation of both Erk1/2 and P38 in primary T cells triggered by anti-CD3/28. Collectively, the active fraction of H.S showed promising immunosuppressive activities both in vitro and in vivo.
Type of Medium:
Online Resource
ISSN:
0192-415X
,
1793-6853
DOI:
10.1142/S0192415X1450058X
Language:
English
Publisher:
World Scientific Pub Co Pte Ltd
Publication Date:
2014
detail.hit.zdb_id:
2095946-1
detail.hit.zdb_id:
193085-0
