In:
Applied Microbiology, American Society for Microbiology, Vol. 26, No. 3 ( 1973-09), p. 327-331
Abstract:
The ability of 88 fungi, which had been obtained as high-potency strains for acid proteinase production, to produce a new type of acid carboxypeptidase (having on optimal pH of about 3 for hydrolysis of benzyloxycarbonyl-glutamyltyrosine) in surface koji culture was determined. Among the aspergilli, substantial amounts of this new acid carboxypeptidase were produced by Aspergillus saitoi, A. usamii, A. awamori, A. inuii , and A. niger . Maximum yields of acid carboxypeptidase per gram of substrate were obtained by submerged culture in a medium containing 0.9% defatted soybean and 0.6% wheat bran. However, the maximum enzyme concentration per milliliter was obtained with a medium containing 3% defatted soybean and 2% wheat bran. The terminal pH could be controlled by varying the concentrations of soybean oil meal and wheat bran. The maximum enzyme production was reached after 4 days or more at 30 C.
Type of Medium:
Online Resource
ISSN:
0003-6919
DOI:
10.1128/am.26.3.327-331.1973
Language:
English
Publisher:
American Society for Microbiology
Publication Date:
1973
detail.hit.zdb_id:
207801-6
detail.hit.zdb_id:
1478346-0
