In:
Infection and Immunity, American Society for Microbiology, Vol. 76, No. 7 ( 2008-07), p. 3214-3220
Abstract:
Oxygenated fatty acids, or oxylipins, play an essential role in physiological signaling and developmental processes in animals, plants, and fungi. Previous characterization of three Aspergillus fumigatus dioxygenases (PpoA, PpoB, and PpoC), similar in sequence to mammalian cyclooxygenases, showed that PpoA is responsible for the production of the oxylipins 8 R -hydroperoxyoctadecadienoic acid and 5 S ,8 R -dihydroxy-9 Z ,12 Z -octadecadienoic acid and that PpoC is responsible for 10 R -hydroxy-8 E ,12 Z -hydroperoxyoctadecadienoic acid. Here, Δ ppo mutants were characterized to elucidate the role of fungal dioxygenases in A. fumigatus development and host interactions. The Δ ppoC strain displayed distinct phenotypes compared to those of other Δ ppo mutants and the wild type, including altered conidium size, germination, and tolerance to oxidative stress as well as increased uptake and killing by primary alveolar macrophages. These experiments implicate oxylipins in pathogen development and suggest that Δ ppoC represents a useful model for studying the A. fumigatus -host interaction.
Type of Medium:
Online Resource
ISSN:
0019-9567
,
1098-5522
DOI:
10.1128/IAI.00009-08
Language:
English
Publisher:
American Society for Microbiology
Publication Date:
2008
detail.hit.zdb_id:
1483247-1
