In:
Applied and Environmental Microbiology, American Society for Microbiology, Vol. 70, No. 12 ( 2004-12), p. 7545-7549
Abstract:
We reevaluated the bias toward a 1:1 ratio of products in multitemplate PCR used in ecological studies and showed that the template reannealing at the annealing step would not cause the bias; however, the preferential homoduplex formation during temperature decrease from denaturation to annealing step would cause the bias.
Type of Medium:
Online Resource
ISSN:
0099-2240
,
1098-5336
DOI:
10.1128/AEM.70.12.7545-7549.2004
Language:
English
Publisher:
American Society for Microbiology
Publication Date:
2004
detail.hit.zdb_id:
223011-2
detail.hit.zdb_id:
1478346-0
SSG:
12