In:
Antimicrobial Agents and Chemotherapy, American Society for Microbiology, Vol. 54, No. 1 ( 2010-01), p. 509-512
Abstract:
We present a simple assay to examine effects of compounds on virulence gene expression in the human pathogen Staphylococcus aureus . The assay employs transcriptional reporter strains carrying lacZ fused to central virulence genes. Compounds affecting virulence gene expression and activity of the agr locus are scored based on color change in the presence of a chromogenic β-galactosidase substrate. The assay can be used to screen for novel antivirulence compounds from many different sources, such as fungi, as demonstrated here.
Type of Medium:
Online Resource
ISSN:
0066-4804
,
1098-6596
DOI:
10.1128/AAC.00940-09
Language:
English
Publisher:
American Society for Microbiology
Publication Date:
2010
detail.hit.zdb_id:
1496156-8
SSG:
12
SSG:
15,3