In:
Science, American Association for the Advancement of Science (AAAS), Vol. 239, No. 4841 ( 1988-02-12), p. 783-785
Abstract:
Mesoderm induction in the amphibian embryo can be studied by exposing animal region explants (destined to become ectoderm) to appropriate stimuli and assaying the appearance of mesodermal products like α-actin messenger RNA. Transforming growth factor β2 (TGF-β2), but not TGF-β1, was active in α-actin induction, while addition of fibroblast growth factor had a small synergistic effect. Medium conditioned by Xenopus XTC cells (XTC-CM), known to have powerful mesoderm-inducing activity, was shown to contain TGF-β-like activity as measured by a radioreceptor binding assay, colony formation in NRK cells, and growth inhibition in CCL64 cells. The activity of XTC-CM in mesoderm induction and in growth inhibition of CCL64 cells was inhibited partially by antibodies to TGF-β2 but not by antibodies to TGF-β1. Thus, a TGF-β2-like molecule may be involved in mesoderm induction.
Type of Medium:
Online Resource
ISSN:
0036-8075
,
1095-9203
DOI:
10.1126/science.3422517
Language:
English
Publisher:
American Association for the Advancement of Science (AAAS)
Publication Date:
1988
detail.hit.zdb_id:
128410-1
detail.hit.zdb_id:
2066996-3
SSG:
11
