In:
Transfusion, Wiley, Vol. 54, No. 4 ( 2014-04), p. 1088-1092
Abstract:
Cryopreservation is often used to store cellular therapies, but little is known about how well CD 3+ or CD 34+ cells tolerate this process. Study Design and Methods Viable CD 34+ cell recoveries were analyzed from related and unrelated donor granulocyte–colony‐stimulating factor ( G ‐ CSF )–mobilized peripheral blood stem cell ( PBSC ) products and viable CD 3+ cell recoveries from G ‐ CSF –mobilized and nonmobilized apheresis products from related and unrelated donors. All products were cryopreserved with 5% dimethyl sulfoxide and 6% pentastarch using a controlled‐rate freezer and were stored in liquid nitrogen. Related donor products were cryopreserved immediately after collection and unrelated donor products greater than 12 hours postcollection. Results The postthaw recovery of CD 34+ cells from related donor PBSC s was high (n = 86; 97.5 ± 23.1%) and there was no difference in postthaw CD 34+ cell recovery from unrelated donor PBSC s (n = 14; 98.8 ± 37.2%; p = 0.863). In related donor lymphocyte products the postthaw CD 3+ cell recovery (n = 48; 90.7 ± 21.4%) was greater than that of unrelated donor products (n = 14; 66.6 ± 35.8%; p = 0.00251). All unrelated donor lymphocyte products were from G ‐ CSF –mobilized products, while most related donor lymphocyte products were from nonmobilized products. A comparison of the CD 3+ cell recovery from related donor G ‐ CSF –mobilized products (n = 19; 85.0 ± 29.2%) with that of unrelated donor products found no significant difference (p = 0.137). Conclusions The postthaw recovery of CD 34+ cells was high in both related and unrelated donor products, but the recovery of CD 3+ cells in unrelated donor G ‐ CSF –mobilized products was lower. G ‐ CSF –mobilized unrelated donor products may contain fewer CD 3+ cells than non– G ‐ CSF –exposed products upon thaw and, when indicated, cell doses should be monitored.
Type of Medium:
Online Resource
ISSN:
0041-1132
,
1537-2995
DOI:
10.1111/trf.2014.54.issue-4
Language:
English
Publisher:
Wiley
Publication Date:
2014
detail.hit.zdb_id:
208417-X
detail.hit.zdb_id:
2018415-3
