In:
Plant Breeding, Wiley, Vol. 134, No. 4 ( 2015-08), p. 400-405
Abstract:
Anthocyanins contribute to plants’ defence against a number of abiotic and biotic stress agents. The anthocyanin pigmentation of the barley leaf sheath is genetically determined by A nt1, a gene which maps to a region of chromosome 7 HS delimited by the microsatellite loci X gbms0226 and X gbms0240 . The sequence of the maize gene C 1 (encoding an R 2 R 3 MYB factor regulating anthocyanin synthesis) was used for the PCR ‐based cloning of A nt1 . In ant1 genotypes, no transcript is generated in the leaf sheath, whereas the gene is active in the presence of the dominant allele. A comparison of the coding and promoter sequences of A nt1 (which induces purple pigmentation in the leaf sheath) and ant1 (which does not) showed that the key polymorphisms lay in the promoter sequence. The transcription of four anthocyanin synthesis structural genes ( C hi , F 3h , D fr , A ns ) was dependent on the allelic status of A nt1 .
Type of Medium:
Online Resource
ISSN:
0179-9541
,
1439-0523
DOI:
10.1111/pbr.2015.134.issue-4
Language:
English
Publisher:
Wiley
Publication Date:
2015
detail.hit.zdb_id:
2020488-7
SSG:
12