In:
Plant Biotechnology Journal, Wiley, Vol. 17, No. 11 ( 2019-11), p. 2143-2152
Abstract:
The fast growth, ease of metabolic labelling and potential for feedstock and biofuels production make duckweeds not only an attractive model system for understanding plant biology, but also a potential future crop. However, current duckweed research is constrained by the lack of efficient genetic manipulation tools. Here, we report a case study on genome editing in a duckweed species, Lemna aequinoctialis , using a fast and efficient transformation and CRISPR /Cas9 tool. By optimizing currently available transformation protocols, we reduced the duration time of Agrobacterium‐ mediated transformation to 5–6 weeks with a success rate of over 94%. Based on the optimized transformation protocol, we generated 15 (14.3% success rate) biallelic La PDS mutants that showed albino phenotype using a CRISPR /Cas9 system. Investigations on CRISPR /Cas9‐mediated mutation spectrum among mutated L. aequinoctialis showed that most of mutations were short insertions and deletions. This study presents the first example of CRISPR /Cas9‐mediated genome editing in duckweeds, which will open new research avenues in using duckweeds for both basic and applied research.
Type of Medium:
Online Resource
ISSN:
1467-7644
,
1467-7652
Language:
English
Publisher:
Wiley
Publication Date:
2019
detail.hit.zdb_id:
2136367-5
SSG:
12