In:
The FEBS Journal, Wiley, Vol. 275, No. 7 ( 2008-04), p. 1510-1517
Abstract:
A novel metallobridged bis(β‐cyclodextrin)s 2 [bis(β‐CD)s 2 ] was synthesized and characterized by means of 1 H NMR, IR, element analysis and redox iodometric titration. The fluorescence of metallobridged bis(β‐CD)s 2 was weak compared with bis(β‐CD)s 1 because of the paramagnetism of copper (II) ions. Glutathione was able to form complexes with copper (II) derived from the metallobridged bis(β‐CD)s 2 . This competitive complexation with copper (II) may lead to a significant fluorescence recovery of the bis(β‐CD)s. Therefore, a rapid and simple spectrofluorimetric method was developed for the determination of glutathione. The analytical application for glutathione was investigated in NaCl/P i (pH 6.00) at room temperature. The linear range of the method was 0.30–20.0 μmol·L −1 with a detection limit of 63.8 nmol·L −1 . There was no interference from the plasma constituents. The proposed method had been successfully used to determine glutathione in human plasma.
Type of Medium:
Online Resource
ISSN:
1742-464X
,
1742-4658
DOI:
10.1111/ejb.2008.275.issue-7
DOI:
10.1111/j.1742-4658.2008.06310.x
Language:
English
Publisher:
Wiley
Publication Date:
2008
detail.hit.zdb_id:
2172518-4
SSG:
12
