In:
Acta Pathologica Microbiologica Scandinavica Series A :Pathology, Wiley, Vol. 92A, No. 1-6 ( 1984-03), p. 461-468
Abstract:
The influence of different fixatives on the antigenicity of alpha‐fetoprotein (AFP), albumin, prealbumin, transferrin, ferritin, human chorionic gonadotropin (HCG), specific pregnancy beta 1 ‐glycoprotein (SP 1 ) and human placental lactogen (HPL) was studied. No single fixative was superior for all the antigens investigated. For intracellular antigens the best all‐round fixatives were, however, 10% buffered formalin, 4% buffered paraformaldehyde, 10% formol‐calcium and ethanol acetic acid (EA), cold processed. The preferable fixatives were for AFP: Bouińs fixative, B‐5 and EA; for albumin: 10% formol‐calcium; for prealbumin: Zambonis fixative and B‐5; for transferrin: EA; for ferritin: 10% buffered formalin, 4% buffered paraformaldehyde, Lillies fixative, Zambonis fixative and EA; for HCG: Bouińs fixative and B‐5; for SP 1 : Lilliés fixative and EA; and for HPL: 4% buffered paraformaldehyde and Lilliés fixative. The preservation of extracellular antigens was different from the above mentioned intracellular ones. Thus fixation in 10% buffered formalin gave poor results while the coagulant fixatives such as Bouins fixative, B‐5 and Clelands fixative in general showed good preservation of the antigenicity of the extracellular antigens.
Type of Medium:
Online Resource
ISSN:
0108-0164
DOI:
10.1111/apm.1984.92A.issue-1-6
DOI:
10.1111/j.1699-0463.1984.tb04428.x
Language:
English
Publisher:
Wiley
Publication Date:
1984
