In:
American Journal of Reproductive Immunology, Wiley, Vol. 42, No. 5 ( 1999-11), p. 263-272
Abstract:
Langer N, Beach D, Lindenbaum ES. Novel hyperactive mitogen to endothelial cells: human decidual NKG5. AJRI 1999; 42:263–272 © Munksgaard, Copenhagen PROBLEM: The purpose of this study was the isolation and characterization of decidual extract proteins that exhibit mitogenicity on endothelial cells. METHOD OF STUDY: A partially purified extract (F 1 fraction) was obtained from human decidua of the first trimester of pregnancy. F 1 was separated by heparin‐sepharose column and showed significant mitogenicity on bovine brain capillary endothelial (BBCE) cells in vitro , using methylene blue stain nuclear assay. Sodium‐dodecyl sulfate polyacrylamide gel electrophoresis (SDS‐PAGE) revealed four fractions with MW less than 30 KDa. RESULTS: Mitogenic fraction, E 11–12 (eluted at 1.5 M NaCl) was identified as basic fibroblast growth factor (bFGF). Vascular endothelial growth factor (VEGF) and acidic (a)FGF were not identified as one of the mitogenic fractions. However, fractions E 5–6 and E 7–8 induced statistically significant mitogenicity at concentration of 1 pg/mL, which is 10 3 times lower than bFGF. Sequencing of E 5–6 fraction identified NKG5 protein, a putative secreted polypeptide derived from human natural killer (NK) cells and activated T cells of unknown function. CONCLUSION: This work demonstrates that NKG5 stimulates mitogenicity of endothelial cells and may be involved in angiogenesis.
Type of Medium:
Online Resource
ISSN:
1046-7408
,
1600-0897
DOI:
10.1111/aji.1999.42.issue-5
DOI:
10.1111/j.1600-0897.1999.tb00100.x
Language:
English
Publisher:
Wiley
Publication Date:
1999
detail.hit.zdb_id:
2024667-5
detail.hit.zdb_id:
604542-X
