In:
European Journal of Oral Sciences, Wiley, Vol. 117, No. 5 ( 2009-10), p. 555-562
Abstract:
4‐Hydroxyphenylacetic acid (HPA) and nitrite are present in human mixed whole saliva, and HPA can be nitrated by peroxidase/hydrogen peroxide (H 2 O 2 )/nitrite systems in the oral cavity. Thus, the objectives of the present study were to estimate the concentrations of HPA, nitrated HPA [4‐hydroxy‐3‐nitrophenylacetic acid (NO 2 HPA)], nitrite, and thiocyanate (SCN − ) in saliva from 73 patients with periodontal diseases and to elucidate the conditions necessary to induce nitration of HPA. High concentrations of HPA, nitrite, and SCN − were found in the saliva of patients older than 50 yr of age. NO 2 HPA was detected in seven patients who were older than 60 yr of age. Nitrite‐dependent formation of NO 2 HPA by a bacterial fraction prepared from mixed whole saliva was faster at pH 5.3 than at pH 7, and increased as the rate of H 2 O 2 formation increased. The formation of NO 2 HPA was inhibited by SCN − and by salivary antioxidants such as uric acid, ascorbic acid, and glutathione. These results suggest that nitration can proceed at an acidic site in the oral cavity where H 2 O 2 is produced under conditions of decreased concentrations of SCN − and of antioxidants.
Type of Medium:
Online Resource
ISSN:
0909-8836
,
1600-0722
DOI:
10.1111/eos.2009.117.issue-5
DOI:
10.1111/j.1600-0722.2009.00671.x
Language:
English
Publisher:
Wiley
Publication Date:
2009
detail.hit.zdb_id:
2025657-7
