In:
Wound Repair and Regeneration, Wiley, Vol. 16, No. 5 ( 2008-09), p. 682-690
Abstract:
Severe injury to the epidermal barrier often results in scarring and life‐long functional deficits, the outcome worsening with a number of factors including time taken to heal. We have investigated the potential of exogenous metallothionein IIA (Zn 7 ‐MT‐IIA), a naturally occurring small cysteine‐rich protein, to accelerate healing of burn wounds in a mouse model. Endogenous MT‐I/II expression increased in basal keratinocytes concurrent with reepithelialization after a burn injury, indicating a role for MT‐I/II in wound healing. In vitro assays of a human keratinocyte cell line indicated that, compared with saline controls, exogenous Zn 7 ‐MT‐IIA significantly increased cell viability by up to 30% ( p 〈 0.05), decreased apoptosis by 13% ( p 〈 0.05) and promoted keratinocyte migration by up to 14% ( p 〈 0.05), all properties that may be desirable to promote rapid wound repair. Further in vitro assays using immortalized and primary fibroblasts indicated that Zn7‐MT‐IIA did not affect fibroblast motility or contraction ( p 〉 0.05). Topical administration of exogenous Zn 7 ‐MT‐IIA (2 μg/mL) in vivo , immediately postburn accelerated healing, promoted faster reepithelialization (3 days: phosphate‐buffered saline (PBS), 8.9±0.3 mm diameter vs. MT‐I/II, 7.1±0.7 mm; 7 days: PBS 5.8±0.98 mm vs. MT‐I/II, 3.6±1.0 mm, p 〈 0.05) and reduced epidermal thickness (MT‐I/II: 45±4 μm vs. PBS: 101±19 μm, p 〈 0.05) compared with controls. Our data suggest that exogenous Zn 7 ‐MT‐IIA may prove a valuable therapeutic for patients with burns and other skin injuries.
Type of Medium:
Online Resource
ISSN:
1067-1927
,
1524-475X
DOI:
10.1111/wrr.2008.16.issue-5
DOI:
10.1111/j.1524-475X.2008.00418.x
Language:
English
Publisher:
Wiley
Publication Date:
2008
detail.hit.zdb_id:
2011990-2
detail.hit.zdb_id:
1174873-4
