In:
Helicobacter, Wiley, Vol. 22, No. 5 ( 2017-10)
Abstract:
Gram‐positive enhancer matrix particles ( GEM ) produced by Lactococcus lactis can enhance vaccine‐induced immune response. However, the mechanism under which this adjuvant mounts the efficacy of orally administered vaccines remains unexplored. Materials and Methods We used a prophylactic mice model to investigate the mechanism of GEM ‐adjuvanted vaccination. Helicobacter pylori urease‐specific antibody response was monitored and detected in murine serum by ELISA . Urease‐specific splenic cytokine profile was examined. Gastric inflammatory responses were measured on day 43 or 71 by quantitative real‐time PCR , flow cytometry and histology. Results We found that GEM enhanced the efficiency of oral H. pylori vaccine by promoting innate immunity. The vaccine CUE ‐ GEM composed of GEM particles and recombinant antigen CTB ‐ UE provided protection of immunized mice against H. pylori insult. The protective response was associated with induction of postimmunization gastritis and local Th1/Th17 cell‐medicated immune response. We showed that innate inflammatory responses including neutrophil chemokines CXCL 1‐2, neutrophils, and antimicrobial proteins S100A8 and MUC 1 were significantly elevated. Within all infected mice, S100A8 and MUC 1 levels were negatively correlated with H. pylori burden. Strikingly, mice receiving GEM also show reduction of colonization, possibly through natural host response pathways to recruit CD 4 + T cells and promote S100A8 expression. Conclusions These findings suggest that GEM ‐based vaccine may impact Th1/Th17 immunity to orchestrate innate immune response against H. pylori infection.
Type of Medium:
Online Resource
ISSN:
1083-4389
,
1523-5378
DOI:
10.1111/hel.2017.22.issue-5
Language:
English
Publisher:
Wiley
Publication Date:
2017
detail.hit.zdb_id:
1330665-0
detail.hit.zdb_id:
2020336-6
SSG:
12
