In:
Clinical Oral Implants Research, Wiley, Vol. 27, No. 12 ( 2016-12)
Abstract:
The peri‐implant epithelium ( PIE ) plays an important role in the prevention against initial stage of inflammation. To minimize the risk of peri‐implantitis, it is necessary to understand the biological characteristics of the PIE . The aim of this study was to investigate the characteristic gene expression profile of PIE as compared to junctional epithelium ( JE ) using laser microdissection and microarray analysis. Methods Left upper first molars of 4‐week‐old rat were extracted, and titanium alloy implants were placed. Four weeks after surgery, samples were harvested by laser microdissection, and total RNA samples were isolated. Comprehensive analyses of genes expressed in the JE and PIE were performed using microarray analysis. Confirmation of the differential expression of selected genes was performed by quantitative real‐time polymerase chain reaction and immunohistochemistry. Results The microarray analysis showed that 712 genes were more than twofold change upregulated in the PIE compared with the JE . Genes S cgb1a1 were significantly upregulated more than 19.1‐fold, L po more than 19.0‐fold, and G bp2 more than 8.9‐fold, in the PIE ( P 〈 0.01). Immunohistochemical localization of SCGB 1 A 1, LPO , and GBP 2 was observed in PIE . Conclusion The present results suggested that genes S cgb1a1, L po, and G bp2 are characteristically expressed in the PIE .
Type of Medium:
Online Resource
ISSN:
0905-7161
,
1600-0501
DOI:
10.1111/clr.2016.27.issue-12
Language:
English
Publisher:
Wiley
Publication Date:
2016
detail.hit.zdb_id:
2027104-9
