In:
Chemical Biology & Drug Design, Wiley, Vol. 82, No. 3 ( 2013-09), p. 296-306
Abstract:
Doxorubicin‐loaded chitosan‐coated superparamagnetic iron oxide nanoparticles ( Fe 3 O 4 ; SPIO‐NP s) were prepared by coprecipitation and emulsification cross‐linking method and uniform NP s with an average particle size of 82 nm, with high encapsulation efficiencies, were obtained. The drug‐loading efficiency of doxorubicin (3.2 mg/mg NP s) showed better results for the chitosan‐loaded SPIO‐NP s as compared to the bare ones (0.5 mg/mg; p 〈 0.05). The incubation of A 2780 and OVCAR ‐3 human ovarian cancer cells with doxorubicin‐loaded and doxorubicin‐loaded chitosan‐coated SPIO‐NP s, for 24, 48, 72, 96, and 120 h, showed significant IC 50 (2.0 ± 0.6 and 7.1 ± 2.7 m m doxorubicin) and IC 90 (4.0 ± 9.2 and 10 ± 0.5 m m doxorubicin), respectively, after 96 h of incubation. While, 95% and 98% growth inhibition was seen in A 2780 and OVCAR ‐3 cells after the 96‐h exposure to the doxorubicin‐chitosan‐ SPIO‐NP s (p 〈 0.05). A 5‐day (120 h) incubation with doxorubicin‐chitosan‐ SPIO‐NP s showed that A 2780 and OVCAR ‐3 cells were able to uptake 120 and 110 pg iron/cell, respectively, when treated with doxorubicin‐chitosan‐ SPIO‐NP s for 72 h (p 〈 0.05).
Type of Medium:
Online Resource
ISSN:
1747-0277
,
1747-0285
DOI:
10.1111/cbdd.2013.82.issue-3
Language:
English
Publisher:
Wiley
Publication Date:
2013
detail.hit.zdb_id:
2216600-2
SSG:
12
