In:
British Journal of Haematology, Wiley, Vol. 175, No. 1 ( 2016-10), p. 102-114
Abstract:
The tumour microenvironment influences outcome in patients with follicular lymphoma ( FL ), but its impact on transformation is less studied. We investigated the prognostic significance of the tumour microenvironment on transformation and survival in FL patients treated in the rituximab era. We examined diagnostic and transformed biopsies from 52 FL patients using antibodies against CD 3, CD 4, CD 8, CD 21 ( CR 2), CD 57 (B3 GAT 1), CD 68, FOXP 3, TIA 1, PD ‐1 ( PDCD 1), PD ‐L1 ( CD 274) and PAX 5. Results were compared with a second cohort of 40 FL patients without signs of transformation during a minimum of five years observation time. Cell numbers and localization were semi‐quantitatively assessed. Better developed CD 21+ follicular dendritic cell ( FDC ) meshworks at diagnosis was a negative prognostic factor for overall survival ( OS ), progression‐free survival ( PFS ) and time to transformation ( TTT ) in patients with subsequently transformed FL . Remnants of FDC meshworks at transformation were associated with shorter OS and PFS from transformation. High degrees of intrafollicular CD 68+ and PD ‐L1+ macrophage infiltration, high total area scores and an extrafollicular/diffuse pattern of FOXP 3+ T cells and high intrafollicular scores of CD 4+ T cells at diagnosis were associated with shorter TTT . Scores of several T‐cell subset markers from the combined patient cohorts were predictive for transformation, especially CD 4 and CD 57.
Type of Medium:
Online Resource
ISSN:
0007-1048
,
1365-2141
DOI:
10.1111/bjh.2016.175.issue-1
Language:
English
Publisher:
Wiley
Publication Date:
2016
detail.hit.zdb_id:
1475751-5