In:
Artificial Organs, Wiley, Vol. 44, No. 10 ( 2020-10), p. 1098-1106
Abstract:
Extracorporeal membrane oxygenation (ECMO) could ameliorate the energy status and viability of bowel grafts from cardiac death donors. However, the function of these grafts after transplantation is not clear. The purpose of the study was to evaluate the early function of intestinal grafts after transplantation from expected cardiac death donors supported with normothermic extracorporeal support using a porcine allogeneic orthotopic segmental small bowel transplantation model. Eighteen domestic crossbred donor pigs were assigned to living donation (LD), donation after cardiac death (DCD), and ECMO groups. In the LD group, small bowels were harvested and preserved immediately in cold storage. In the other two groups, the donor pigs received conventional rapid recovery treatment or 1‐hour normothermic extracorporeal support after 10‐minutes expected cardiac arrest. Subsequently, the small bowels were removed and preserved in cold storage. After 5‐6 hours of preservation, small bowel grafts were transplanted into the recipient pigs that underwent enterectomy. The pathology and electron microscopy results, cell apoptosis rate, tight junction protein expression level in the intestinal mucosa, and plasma endotoxin level were evaluated after transplantation. All grafts functioned on the basis of the maltose absorption test results at day 7 after transplantation. There were no significant differences in the morphological changes in the intestinal mucosa among the three groups at day 7 after transplantation. The cell apoptosis rate and plasma endotoxin level in the ECMO group did not differ significantly than those in the LD group, but were evidently lower than those in the DCD group ( P 〈 .001). The intestinal absorptive function improved significantly in the ECMO group in contrast with that in the DCD group ( P 〈 .001). Short‐term ECMO intervention can alleviate ischemia–reperfusion injuries in intestinal grafts and improve intestinal absorptive function in the early stage after transplantation. Reducing caspase‐3 protein expression and cell apoptosis in the intestinal mucosa may be one of the protective mechanisms of ECMO intervention.
Type of Medium:
Online Resource
ISSN:
0160-564X
,
1525-1594
Language:
English
Publisher:
Wiley
Publication Date:
2020
detail.hit.zdb_id:
2003825-2
