In:
Allergy, Wiley, Vol. 74, No. 4 ( 2019-04), p. 685-697
Abstract:
Chitinase 3‐like 1 protein ( CHI 3L1) ( YKL ‐40 in humans and breast regression protein [ BRP ]‐39 in mice) is required for optimal allergen sensitization and Th2 inflammation in various chronic inflammatory diseases including asthma. However, the role of CHI 3L1 in airway inflammation induced by respiratory viruses has not been investigated. The aim of this study was to investigate the relationship between CHI 3L1 and airway inflammation caused by respiratory syncytial virus ( RSV ) infection. Methods We measured YKL ‐40 levels in human nasopharyngeal aspirate ( NPA ) from hospitalized children presenting with acute respiratory symptoms. Wild‐type ( WT ) and BRP ‐39 knockout ( KO ) C57 BL /6 mice were inoculated with live RSV (A2 strain). Bronchoalveolar lavage fluid and lung tissue samples were obtained on day 7 after inoculation to assess lung inflammation, airway reactivity, and expression of cytokines and BRP ‐39. Results In human subjects, YKL ‐40 and IL ‐13 levels in NPA were higher in children with RSV infection than in control subjects. Expression of BRP ‐39 and Th2 cytokines, IL ‐13 in particular, was increased following RSV infection in mice. Airway inflammation caused by RSV infection was reduced in BRP ‐39 KO mice as compared to WT mice. Th2 cytokine levels were not increased in the lungs of RSV ‐infected BRP ‐39 KO mice. BRP ‐39 regulated M2 macrophage activation in RSV ‐infected mice. Additionally, treatment with anti‐ CHI 3L1 antibody attenuated airway inflammation and Th2 cytokine production in RSV ‐infected WT mice. Conclusion These findings suggest that CHI 3L1 could contribute to airway inflammation induced by RSV infection. CHI 3L1 could be a potential therapeutic candidate for attenuating Th2‐associated immunopathology during RSV infection.
Type of Medium:
Online Resource
ISSN:
0105-4538
,
1398-9995
DOI:
10.1111/all.2019.74.issue-4
Language:
English
Publisher:
Wiley
Publication Date:
2019
detail.hit.zdb_id:
2003114-2
detail.hit.zdb_id:
391933-X
