In:
International Journal of Rheumatic Diseases, Wiley, Vol. 19, No. 10 ( 2016-10), p. 968-973
Abstract:
The purpose of this study is to investigate the role of methylation in the histone deacetylases 6 ( HDAC 6 ) promoter and HDAC 6 messenger RNA ( mRNA ) expression in the pathogenesis of systemic lupus erythematosus ( SLE ). Method Direct bisulfite‐polymerase chain reaction ( PCR ) sequencing was performed to detect the HDAC 6 promoter methylation in 33 patients with SLE and 35 healthy controls. The HDAC 6 mRNA expression was measured in 93 SLE patients and 84 healthy controls by using the method of quantitative real‐time PCR . Results This study demonstrated that the methylation rates at HDAC 6‐680, ‐660 and ‐658 were significantly increased in the SLE patients compared with healthy controls ( P = 0.041, 0.034 and 0.029, respectively). The SLE patients also had lower HDAC 6 mRNA expression than the controls ( P = 0.031). However, there was no significant difference in HDAC 6 mRNA expression between patients with active and inactive SLE. Conclusion The SLE patients had higher methylation in the HDAC 6 promoter and lower HDAC 6 mRNA expression than the controls. These changes may be related to the susceptibility of SLE . However, they are not associated with the disease activity of SLE .
Type of Medium:
Online Resource
ISSN:
1756-1841
,
1756-185X
DOI:
10.1111/apl.2016.19.issue-10
DOI:
10.1111/1756-185X.12783
Language:
English
Publisher:
Wiley
Publication Date:
2016
detail.hit.zdb_id:
2427877-4
