In:
Environmental Microbiology, Wiley, Vol. 22, No. 7 ( 2020-07), p. 2829-2842
Abstract:
Emergence and expansion of frequent multidrug‐resistant (MDR) major Salmonella clones/serotypes has been a significant threat in the last years. Metal compounds, such as copper, commonly used in animal‐production settings, have been pointed out as possible contributors for the selection of such strains/clones. However, the scarcity of studies limits our understanding of the impact of other metal environmental contaminants as arsenic (used in insecticides/herbicides/coccidiostats). We analysed arsenic tolerance (AsT) dispersion by phenotypic and genotypic (PCR/sequencing/I‐ Ceu I/S1/ Xba I‐PFGE/hybridization) assays among Salmonella with diverse epidemiological and genetic backgrounds. Then, to better understand ars operon genetic contexts, the whole genome of five representative strains was sequenced. We found a high dispersion of ars operons conferring AsT, especially among copper‐tolerant and relevant serotypes/clones related to pig‐production setting. The acr3 ‐type was found dispersed in the chromosome of diverse serotypes, including the emergent S . Rissen. Conversely, arsB II was almost confined to the MDR ST34 European clone of S . Typhimurium/ S . 4,[5],12:i:‐, always along with copper/silver tolerance sil + pco clusters in an integrative conjugative element. These data suggest that AsT is an essential adaptive feature for the ecological success of these epidemic clones/serotypes and alerts for global strategies to reduce arsenic‐based compounds' impact thus preventing environmental/food contamination with frequent MDR foodborne pathogens.
Type of Medium:
Online Resource
ISSN:
1462-2912
,
1462-2920
DOI:
10.1111/1462-2920.15016
Language:
English
Publisher:
Wiley
Publication Date:
2020
detail.hit.zdb_id:
2020213-1
SSG:
12
