In:
Environmental Microbiology, Wiley, Vol. 17, No. 9 ( 2015-09), p. 3233-3250
Abstract:
Aerobic methane ( CH 4 ) oxidation mitigates CH 4 release and is a significant pathway for carbon and energy flow into aquatic food webs. Arctic lakes are responsible for an increasing proportion of global CH 4 emissions, but CH 4 assimilation into the aquatic food web in arctic lakes is poorly understood. Using stable isotope probing ( SIP ) based on phospholipid fatty acids ( PLFA‐SIP ) and DNA ( DNA‐SIP ), we tracked carbon flow quantitatively from CH 4 into sediment microorganisms from an arctic lake with an active CH 4 seepage. When 0.025 mmol CH 4 g −1 wet sediment was oxidized, approximately 15.8–32.8% of the CH 4 ‐derived carbon had been incorporated into microorganisms. This CH 4 ‐derived carbon equated to up to 5.7% of total primary production estimates for Alaskan arctic lakes. Type I methanotrophs, including Methylomonas , Methylobacter and unclassified Methylococcaceae , were most active at CH 4 oxidation in this arctic lake. With increasing distance from the active CH 4 seepage, a greater diversity of bacteria incorporated CH 4 ‐derived carbon. Actinomycetes were the most quantitatively important microorganisms involved in secondary feeding on CH 4 ‐derived carbon. These results showed that CH 4 flows through methanotrophs into the broader microbial community and that type I methanotrophs, methylotrophs and actinomycetes are important organisms involved in using CH 4 ‐derived carbon in arctic freshwater ecosystems.
Type of Medium:
Online Resource
ISSN:
1462-2912
,
1462-2920
DOI:
10.1111/emi.2015.17.issue-9
DOI:
10.1111/1462-2920.12773
Language:
English
Publisher:
Wiley
Publication Date:
2015
detail.hit.zdb_id:
2020213-1
SSG:
12
