In:
Journal of Applied Crystallography, International Union of Crystallography (IUCr), Vol. 43, No. 5 ( 2010-10-01), p. 1021-1026
Abstract:
This paper presents a modification to the conventional vapor diffusion (hanging- or sitting-drop) technique for protein crystallization screening. In this modified method, the reservoir solution is replaced with a desiccant to allow for a larger range of protein solution concentrations, thereby providing more opportunities for crystal formation. This method was tested in both reproducibility and screening studies, and the results showed that it significantly improves the efficiency and reduces the cost of protein crystallization screens.
Type of Medium:
Online Resource
ISSN:
0021-8898
DOI:
10.1107/S0021889810030803
DOI:
10.1107/S0021889810030803/he5489sup1.pdf
Language:
Unknown
Publisher:
International Union of Crystallography (IUCr)
Publication Date:
2010
detail.hit.zdb_id:
2020879-0