In:
International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 72, No. 4 ( 2022-04-08)
Abstract:
A novel bacterium, designated SCR006 T , was isolated from tidal flat sediment from Suncheon Bay, Republic of Korea. Cells of strain SCR006 T were strictly anaerobic, motile cocci, Gram-reaction-negative, and catalase- and oxidase-negative. Growth was observed at 4–41 °C (optimum, 34–37 °C), at pH 6.5–10.0 (optimum, pH 7.0–7.5) and in presence of 0–8 % NaCl (optimum, 0–2 %). Fermentation products of peptone–yeast–glucose medium were acetate and ethanol. Results of phylogenetic analyses based on 16S rRNA gene sequences indicated that strain SCR006 T had high sequence similarity to Proteiniclasticum ruminis D3RC-2 T (97.9 %), followed by Youngiibacter multivorans DSM 6139 T (95.9 %) and Youngiibacter fragilis 232.1 T (95.0 %). The average nucleotide identity value between strain SCR006 T and P. ruminis DSM 24773 T was 72.7 %, which strongly supported that strain SCR006 T reresents a novel species within the genus Proteiniclasticum . The major cellular fatty acids are iso-C 15 : 0 (27.2 %) and anteiso-C 15 : 0 (16.9 %). The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, two unidentified phospholipids, an unidentified aminolipid and five unidentified lipids. The genomic size was 3.2 Mb with genomic DNA G+C content of 45.6 mol%. The results of 16S rRNA-based and genome-based phylogenetic tree analyses indicated that SCR006 T should be assigned to the genus Proteiniclasticum . Strain SCR006 T could be distinguished from P. ruminis D3RC-2 T by its growth conditions, cell morphology and genomic characteristics. Based on the phenotypic, phylogenetic, genomic and chemotaxonomic features, strain SCR006 T represents a novel species, for which the name Proteiniclasticum aestuarii sp. nov. is proposed, with the type strain SCR006 T (=KCTC 25245 T = JCM 34531 T )
Type of Medium:
Online Resource
ISSN:
1466-5026
,
1466-5034
DOI:
10.1099/ijsem.0.005275
Language:
English
Publisher:
Microbiology Society
Publication Date:
2022
detail.hit.zdb_id:
215062-1
detail.hit.zdb_id:
2056611-6
SSG:
12
