In:
International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 72, No. 2 ( 2022-02-10)
Abstract:
Three Gram-staining-negative, aerobic and rod-shaped strains, designated as T40-1 T , T40-3 T and JL-62 T , were isolated from the deep-sea water in the southwest Indian ridge. For strain T40-1 T , growth occurred at 15–37 °C (optimum, 28 °C), pH 6.0–9.0 (optimum, pH 7.5) and in the presence of 0.5–5.0 % NaCl (w/v; optimum, 2.0 %). Strain T40-3 T could grow at 15–40 °C (optimum, 28 °C), with 0.5–11.0 % NaCl (optimum, 2.0 %, w/v) at pH 6.0–9.5 (optimum, 8.0). The temperature, pH and salinity ranges for growth of strain JL-62 T were 15–40 °C (optimum, 30 °C), pH 5.5–9.0 (optimum, pH 7.5–8.0) and 0.5–9.0 % NaCl (w/v; optimum, 4.0 %). Ubiquinone-10 was the sole ubiquinone in all strains, the major fatty acids ( 〉 20 %) were summed feature 8 (C 18 : 1 ω 7 c / C 18 : 1 ω 6 c ). The major polar lipids of strains T40-1 T and T40-3 T were phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine and diphosphatidylglycerol. Strain JL-62 T contained phosphatidylmonomethylethanolamine, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and sulfoquinovosyldiacylglycerol as major polar lipids. Phylogenetic trees based on 16S rRNA gene and core-genomic sequences revealed affiliation of strains T40-1 T and T40-3 T to the family Roseobacteraceae and formed two independent clades from other Roseobacteraceae genera, and those two strains had average nucleotide identities of 62.0–72.0 % to their phylogenetically related species which fell into to the genus boundary range, indicating that they represent two novel genera. While strain JL-62 T represents a novel species in the genus Oricola belonging to the family Phyllobacteriaceae , which was supported by overall genomic relatedness index calculations. The DNA G+C contents of strains T40-1 T , T40-3 T and JL-62 T were 66.5, 60.1 and 62.1 mol %, respectively. Based on the polyphasic taxonomic data, strains T40-1 T (=MCCC M24557 T =KCTC 82975 T ) and T40-3 T (=MCCC 1K05135 T =KCTC 82976 T ) are classified as representing two novel genera belonging to the family Roseobacteraceae with the names Mesobacterium pallidum gen. nov., sp. nov. and Heliomarina baculiformis gen. nov., sp. nov. are proposed, and strain JL-62 T (=MCCC M24579 T =KCTC 82974 T ) is proposed to represent a novel species within the genus Oricola with the name Oricola indica sp. nov. is proposed.
Type of Medium:
Online Resource
ISSN:
1466-5026
,
1466-5034
DOI:
10.1099/ijsem.0.005236
Language:
English
Publisher:
Microbiology Society
Publication Date:
2022
detail.hit.zdb_id:
215062-1
detail.hit.zdb_id:
2056611-6
SSG:
12
