In:
International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 64, No. Pt_8 ( 2014-08-01), p. 2593-2598
Abstract:
A novel aerobic, halotolerant bacterium, designated strain LAM612 T , was isolated from saline-alkaline soil samples from Lingxian County, Shandong Province, China. Cells of strain LAM612 T were Gram-reaction-positive, endospore-forming, motile and rod-shaped. The optimal temperature and pH for growth were 35 °C and pH 6.0, respectively. Strain LAM612 T could grow in the presence of up to 10 % (w/v) NaCl. The genomic DNA G+C conten was 36.4 mol% as detected by the T m method. Comparative analysis of 16S rRNA gene sequences revealed that LAM612 T was closely related to Lysinibacillus sinduriensis KACC 16611 T (98.0 %), L. chungkukjangi KACC 16626 T (97.5 %), L. massiliensis KCTC 13178 T (97.4 %), L. xylanilyticus KACC 15113 T (97.2 %), L. macroides DSM 54 T (97.0 %) and L. manganicus DSM 26584 T (96.5 %). The DNA–DNA hybridization values between strain LAM612 T and its closest relatives ranged from 20.6 % to 41.9 %. The major fatty acids of strain LAM612 T were iso-C 15 : 0 (40.8 %), iso-C 16 : 0 (15.2 %) and anteiso-C 15 : 0 (10.8 %). The cell-wall peptidoglycan content was A4α ( l -Lys– d -Asp). The predominant menaquinone was MK-7 and the main polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, three unknown phospholipids, five unknown glycolipids and an unknown lipid. Based on the DNA–DNA hybridization results and phenotypic, phylogenetic and chemotaxonomic properties, strain LAM612 T could be distinguished from the recognized species of the genus Lysinibacillus , and was suggested to represent a novel species of this genus, for which the name Lysinibacillus halotolerans sp. nov. is proposed. The type strain is LAM612 T ( = ACCC 00718 T = JCM 19611 T ).
Type of Medium:
Online Resource
ISSN:
1466-5026
,
1466-5034
DOI:
10.1099/ijs.0.061465-0
Language:
English
Publisher:
Microbiology Society
Publication Date:
2014
detail.hit.zdb_id:
215062-1
detail.hit.zdb_id:
2056611-6
SSG:
12
