In:
International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 63, No. Pt_12 ( 2013-12-01), p. 4591-4598
Abstract:
An aerobic, Gram-stain-negative, rod-shaped bacterium (designated strain CC-G9A T ), motile by a polar-flagellum, was isolated from a hot spring water sample in Taiwan. Strain CC-G9A T could grow at 20–42 °C, pH 6.0–10.0 and tolerate up to 7 % (w/v) NaCl. The 16S rRNA gene sequence analysis of strain CC-G9A T showed pairwise sequence similarity to Pseudomonas mendocina LMG 1223 T (97.7 %), Pseudomonas alcaligenes ATCC 14909 T (97.8 %), Pseudomonas alcaliphila DSM 17744 T (97.8 %), Pseudomonas toyotomiensis JCM 15604 T (97.6 %), Pseudomonas oleovorans subsp. lubricantis DSM 21016 T (97.6 %) and Pseudomonas argentinensis BCRC 17807 T (97.5 %), and lower sequence similarity to other species of the genus Pseudomonas . According to DNA–DNA association analysis, the relatedness of strain CC-G9A T to P. mendocina BCRC 10458 T , P. alcaliphila DSM 17744 T , P. alcaligenes BCRC 11893 T , P. oleovorans subsp. lubricantis DSM 21016 T , P. argentinensis BCRC 17807 T and P. oleovorans subsp. oleovorans BCRC 11902 was 55.1±3.1, 13.7±1.5, 14.1±1.8, 58.5±1.1, 28.9±2.0 and 28.6±1.8 %, respectively. The evolutionary trees reconstructed based on 16S rRNA, gyrB and rpoB gene sequences revealed varying phylogenetic neighbourhoods of strain CC-G9A T with regard to the most closely related type strains. The predominant quinone system was ubiquinone (Q-9) and the DNA G+C content was 64.3±1.3 mol%. The major fatty acids were C 10 : 0 3-OH, C 12 : 0, C 12 : 0 3-OH, C 16 : 0 and summed features 3 and 8 consisting of C 16 : 1 ω7 c /C 16 : 1 ω6 c and C 18 : 1 ω7 c /C 18 : 1 ω6 c , respectively. The major polar lipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine and phosphatidylglycerol. According to distinct phylogenetic, phenotypic and chemotaxonomic features, strain CC-G9A T is proposed to represent a novel species within the genus Pseudomonas for which the name Pseudomonas guguanensis sp. nov. is proposed. The type strain is CC-G9A T ( = BCRC 80438 T = JCM 18416 T ).
Type of Medium:
Online Resource
ISSN:
1466-5026
,
1466-5034
DOI:
10.1099/ijs.0.047712-0
Language:
English
Publisher:
Microbiology Society
Publication Date:
2013
detail.hit.zdb_id:
215062-1
detail.hit.zdb_id:
2056611-6
SSG:
12
