In:
Journal of Antimicrobial Chemotherapy, Oxford University Press (OUP), Vol. 77, No. 11 ( 2022-10-28), p. 2960-2963
Abstract:
To investigate the fitness effects of large blaCTX-M-15-harbouring F2:A1:B− plasmids on their native Escherichia coli ST131 H30Rx hosts. Methods We selected five E. coli ST131 H30Rx isolates of diverse origin, each carrying an F2:A1:B− plasmid with the blaCTX-M-15 gene. The plasmid was eliminated from each isolate by displacement using an incompatible curing plasmid, pMDP5_cureEC958. WGS was performed to obtain complete chromosome and plasmid sequences of original isolates and to detect chromosomal mutations in ‘cured’ clones. High-throughput competition assays were conducted to determine the relative fitness of cured clones compared with the corresponding original isolates. Results We were able to successfully eliminate the F2:A1:B− plasmids from all five original isolates using pMDP5_cureEC958. The F2:A1:B− plasmids produced non-significant fitness effects in three isolates and moderate reductions in relative fitness (3%–4%) in the two remaining isolates. Conclusions We conclude that F2:A1:B− plasmids pose low fitness costs in their E. coli ST131 H30Rx hosts. This plasmid-host fitness compatibility is likely to promote the maintenance of antibiotic resistance in this clinically important E. coli lineage.
Type of Medium:
Online Resource
ISSN:
0305-7453
,
1460-2091
Language:
English
Publisher:
Oxford University Press (OUP)
Publication Date:
2022
detail.hit.zdb_id:
1467478-6
SSG:
15,3
