In:
Proceedings of the National Academy of Sciences, Proceedings of the National Academy of Sciences, Vol. 79, No. 13 ( 1982-07), p. 4161-4165
Abstract:
Large numbers of Thy-1-positive cells were observed in cultures of bone marrow cells that had been depleted of T cells and grown for 3 to 4 days in the presence of medium conditioned by concanavalin A-activated spleen cells. Cells bearing levels of Thy-1 comparable with those on the bulk of thymocytes were isolated by using the fluorescence-activated cell sorter. Although many were large blasts, the Thy-1-positive cells failed to grow in response to T-cell growth factor and concanavalin A; about one-third, however, proliferated in the presence of factors stimulating hemopoietic progenitor cells. Furthermore, the Thy-1-positive population included cells capable of forming large colonies of macrophages and granulocytes in agar and cells forming splenic colonies in lethally irradiated mice. The appearance of the Thy-1-positive cells did not correlate with the presence of either T-cell growth factor or T-cell-derived granulocyte/macrophage colony-stimulating factor. These findings indicate that Thy-1 can occur on various murine hemopoietic stem and progenitor cells and myeloid cells; Thy-1 can no longer be regarded as an unambiguous marker of commitment to the T-cell lineage.
Type of Medium:
Online Resource
ISSN:
0027-8424
,
1091-6490
DOI:
10.1073/pnas.79.13.4161
Language:
English
Publisher:
Proceedings of the National Academy of Sciences
Publication Date:
1982
detail.hit.zdb_id:
209104-5
detail.hit.zdb_id:
1461794-8
SSG:
11
SSG:
12
