In:
Proceedings of the National Academy of Sciences, Proceedings of the National Academy of Sciences, Vol. 98, No. 20 ( 2001-09-25), p. 11604-11608
Abstract:
In addition to essential nutrients, human milk contains
several classes of bioactive factors such as enzymes, hormones, and growth factors, many of which are implicated in infantile growth and
development. Secretory carbonic anhydrase isoenzyme VI (CA VI) has been identified earlier as an essential component of mammalian saliva, and
we demonstrate here by using biochemical and immunohistochemical techniques that it is also an elementary component of milk. The 42-kDa
glycopolypeptide purified from human milk in CA inhibitor affinity chromatography shared 100% homology with salivary CA VI in the protein
sequence analysis (40% coverage), and its digestion with PNGase F resulted in a polypeptide backbone similar in size to salivary CA VI.
Quantification of CA VI in milk by using a time-resolved immunofluorometric assay revealed an approximately eight-times-higher
concentration in human colostrum than in mature milk, the latter corresponding to the levels previously detected in human saliva. The
high concentration in the colostrum, in particular its functional and structural stability in an acidic milieu, and its growth-supporting
role in the taste buds suggest that milk CA VI is an essential factor in normal growth and development of the infant alimentary tract.
Type of Medium:
Online Resource
ISSN:
0027-8424
,
1091-6490
DOI:
10.1073/pnas.121172598
Language:
English
Publisher:
Proceedings of the National Academy of Sciences
Publication Date:
2001
detail.hit.zdb_id:
209104-5
detail.hit.zdb_id:
1461794-8
SSG:
11
SSG:
12
