In:
Reproduction, Fertility and Development, CSIRO Publishing, Vol. 24, No. 1 ( 2012), p. 231-
Abstract:
The ubiquitin (ub)-mediated degradation of certain regulatory proteins plays critical roles in various functions, including cell cycle, signal transduction, transcription regulation and endocytosis. Usp9x is stage dependently expressed in the germ cells during mouse gametogenesis. Af-6, a cell junction protein, has been identified as a substrate of Usp9x, indicating a possible association between Usp9x and Af-6 in spermatogenesis and oogenesis. In this study, we examined the expression patterns of Usp9x and Af-6 and their intracellular localization in transgenic pig testes and ovary. In both Sertoli and granulosa cells, Af-6 was continuously expressed throughout postnatal and adult stages. Both Af-6 and Usp9x were enriched at the sites of Sertoli-spermatid junctions, especially at stage IV. In granulosa cells, Usp9x was strongly expressed in primary follicles, but its expression rapidly decreased after the late-secondary follicle stage. On the other hand, the expression of both Usp9x and Af-6 was weak in transgenic pigs, with low expression in the Sertoli cells and relatively stronger expression in Graafian follicles. This study suggests that Usp9x and Af-6 were deubiquitylated in Sertoli and follicle cells in wild type and transgenic pigs.
This study was carried out with the support of Agenda Program (Project No. PJ006702), RDA, Republic of Korea.
Type of Medium:
Online Resource
ISSN:
1031-3613
DOI:
10.1071/RDv24n1Ab237
Language:
English
Publisher:
CSIRO Publishing
Publication Date:
2012
SSG:
12
