In:
Biochemical Journal, Portland Press Ltd., Vol. 186, No. 1 ( 1980-01-15), p. 177-181
Abstract:
A method was developed for the analytical and preparative isolation of basolateral plasma membranes from rat small intestine. They were separated on a self-orientating Percoll (modified colloidal silica) gradient starting with a heavy microsomal-membrane fraction and involving centrifugation at 48,000 g for 1 h. (Na+ + K+)-stimulated ATPase activity, used as a marker enzyme for the basolateral plasma membrane, is enriched 20-fold compared with that found in the homogenate of isolated intestinal epithelial cells.
Type of Medium:
Online Resource
ISSN:
0264-6021
Language:
English
Publisher:
Portland Press Ltd.
Publication Date:
1980
detail.hit.zdb_id:
1473095-9
SSG:
12