In:
Analytical Methods, Royal Society of Chemistry (RSC), Vol. 15, No. 15 ( 2023), p. 1855-1860
Abstract:
Voltage-gated sodium channels (VGSCs) are widely expressed in various types of tumor and cancer cells, and Na V 1.5 is overexpressed in highly metastatic breast cancer cells. There may be positive relations between the expression levels of Na V 1.5 and breast cancer recurrence and metastasis. Herein, Na V 1.5 was detected and localized on the surfaces of normal and cancer breast cells by the single molecule recognition imaging (SMRI) mode of atomic force microscopy (AFM). The results reveal that Na V 1.5 was irregularly distributed on the surfaces of normal and cancer breast cells. The Na V 1.5 has an area percentage of 0.6% and 7.2% on normal and cancer breast cells, respectively, which indicates that there is more Na V 1.5 on cancer cells than on normal cells. The specific interaction forces and binding kinetics in the Na V 1.5–antibody complex system were investigated with the single molecule force spectroscopy (SMFS) mode of AFM, indicating that the stability of the Na V 1.5–antibody on normal breast cells is higher than that on cancer breast cells. All these results will be useful to study the interactions of other ion channel–antibody systems, and will also be useful to understand the role of sodium channels in tumor metastasis and invasion.
Type of Medium:
Online Resource
ISSN:
1759-9660
,
1759-9679
Language:
English
Publisher:
Royal Society of Chemistry (RSC)
Publication Date:
2023
detail.hit.zdb_id:
2515210-5
